Early Detection of Mycoplasma Gallisepticum using PCR from Field Samples in Layer Chicken Authors: Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain & Mir Rawshan Akter

Page: 699-706

Early Detection of  Mycoplasma  Gallisepticum  using PCR from  Field  Samples  in Layer  Chicken

Authors: 

Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain & Mir Rawshan Akter

Abstract

Mycoplasma gallisepticum (MG) is an important avian pathogen which causes significant economic losses within layer poultry industry. To control of this economic losses, early and specific detection is necessary. A total of 48 (tracheal swabs, lungs and air sacs) samples were collected from different infected/suspected flocks causing respiratory distress. DNA was extracted and then, 16sRNA gene was amplified by PCR using species specific primers and identified 6 positive samples (12.5%). MG exhibited from tracheal swab, lung and air sac were 25, 6.25 and 6.25% respectively. Based on result, it is concluded that PCR using tracheal swab can be efficient for rapid screening and early detection of MG in layer chicken.

. Key words: Mycoplasma gallisepticum, Diagnosis, 16S rRNA, PCR, Chicken

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Title:

Early Detection of  Mycoplasma  Gallisepticum  using PCR from  Field  Samples  in Layer  Chicken

Author:

Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain & Mir Rawshan Akter

Journal Name: International Journal of Science and Business
Website: ijsab.com
ISSN: ISSN 2520-4750 (Online), ISSN 2521-3040 (Print)
DOI: https://doi.org/10.5281/zenodo.1486075
Media: Print & Online
Volume: 2
Issue: 4
Acceptance Date: 11/11/2018
Date of Publication: 13/11/2018
PDF URL: http://ijsab.com/wp-content/uploads/288.pdf
Free download: Available
Page: 699-706
First Page: 699
Last Page: 706
Current Status: Published

Cite This Article:

Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan,  Md. Khaled Hossain, & Mir Rawshan Akter (2018). Early Detection of  Mycoplasma  Gallisepticum  using PCR from  Field  Samples  in Layer  Chicken. International Journal of Science and Business, 2(4), 699-706. doi: https://doi.org/10.5281/zenodo.1486075

Retrieved from http://ijsab.com/wp-content/uploads/288.pdf

 

About Author

Mahfuja khatun, (Corresponding Author) Department of Livestock Services, Bangladesh.

Md. Mostafizer Rahman, Professor, Department of Microbiology, Hajee Mohammad Danesh science and Technology  University, Dinajpur, Bangladesh.

Md. Shahidur Rahman Khan, Professor, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Md. Khaled Hossain, Professor, Department of Microbiology, Hajee Mohammad Danesh science and Technology University,  Dinajpur, Bangladesh.

Mir Rawshan Akter, Professor, Department of Microbiology, Hajee Mohammad Danesh science and Technology University, Dinajpur, Bangladesh.

 

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DOI: https://doi.org/10.5281/zenodo.1486075

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