Early Detection of Mycoplasma Gallisepticum using PCR from Field Samples in Layer Chicken
Authors:
Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain & Mir Rawshan Akter
Abstract
Mycoplasma gallisepticum (MG) is an important avian pathogen which causes significant economic losses within layer poultry industry. To control of this economic losses, early and specific detection is necessary. A total of 48 (tracheal swabs, lungs and air sacs) samples were collected from different infected/suspected flocks causing respiratory distress. DNA was extracted and then, 16sRNA gene was amplified by PCR using species specific primers and identified 6 positive samples (12.5%). MG exhibited from tracheal swab, lung and air sac were 25, 6.25 and 6.25% respectively. Based on result, it is concluded that PCR using tracheal swab can be efficient for rapid screening and early detection of MG in layer chicken.
. Key words: Mycoplasma gallisepticum, Diagnosis, 16S rRNA, PCR, Chicken
| Title: |
Early Detection of Mycoplasma Gallisepticum using PCR from Field Samples in Layer Chicken |
|---|---|
| Author: |
Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain & Mir Rawshan Akter |
| Journal Name: | International Journal of Science and Business |
| Website: | ijsab.com |
| ISSN: | ISSN 2520-4750 (Online), ISSN 2521-3040 (Print) |
| DOI: | https://doi.org/10.5281/zenodo.1486075 |
| Media: | Print & Online |
| Volume: | 2 |
| Issue: | 4 |
| Acceptance Date: | 11/11/2018 |
| Date of Publication: | 13/11/2018 |
| PDF URL: | https://ijsab.com/wp-content/uploads/288.pdf |
| Free download: | Available |
| Page: | 699-706 |
| First Page: | 699 |
| Last Page: | 706 |
| Current Status: | Published |
Cite This Article:
Mahfuja khatun, Md. Mostafizer Rahman, Md. Shahidur Rahman Khan, Md. Khaled Hossain, & Mir Rawshan Akter (2018). Early Detection of Mycoplasma Gallisepticum using PCR from Field Samples in Layer Chicken. International Journal of Science and Business, 2(4), 699-706. doi: https://doi.org/10.5281/zenodo.1486075
Retrieved from https://ijsab.com/wp-content/uploads/288.pdf
About Author
Mahfuja khatun, (Corresponding Author) Department of Livestock Services, Bangladesh.
Md. Mostafizer Rahman, Professor, Department of Microbiology, Hajee Mohammad Danesh science and Technology University, Dinajpur, Bangladesh.
Md. Shahidur Rahman Khan, Professor, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Md. Khaled Hossain, Professor, Department of Microbiology, Hajee Mohammad Danesh science and Technology University, Dinajpur, Bangladesh.
Mir Rawshan Akter, Professor, Department of Microbiology, Hajee Mohammad Danesh science and Technology University, Dinajpur, Bangladesh.
DOI: https://doi.org/10.5281/zenodo.1486075